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Validation of new physiological targets of peroxisome-derived hydrogen peroxide

Reversible oxidative protein modifications allow cells to respond rapidly to a multitude of physiological and environmental stimuli. Accumulating evidence indicates that peroxisomes have the intrinsic ability to mediate and modulate redox-driven signaling events. However, the molecular mechanisms and physiological relevance of these events are only poorly understood.

The overall aim of this project is to improve our understanding of the molecular targets and mechanisms involved in peroxisomal H2O2 signaling. Therefore, we employ a thiol-based redox proteomics approach to identify and compare proteins that can be oxidatively modified by peroxisome-derived H2O2.

The aim of this study is to validate a number of the targets identified (typical targets include transcription factors, kinases, phosphatases, and antioxidant enzymes).

The outcome of this basic knowledge-driven project is a first essential step in understanding to what extent and through which mechanisms alterations in peroxisomal H2O2 metabolism contribute to cellular aging and the development of age-associated diseases.

  • The research project is available in the Fall and Spring semester.
  • The reserch project is also open for recently graduated undergraduate students.
  • Number of placements available: 1 per semester.

Prerequisites

  • Strong basic knowledge in molecular cell biology.
  • Strong interest in organelle biology.

Faculty Department

Faculty of MedicineDepartment of Cellular and Molecular Medicine

The Department of Cellular and Molecular Medicine combines expertises in techniques of:

  • biochemistry
  • electrophysiology
  • molecular biology
  • cell imaging
  • proteomics
  • bioinformatics
  • animal-model development

to acquire novel insights into cellular signaling and communication processes.

An additional aim is to decipher the molecular basis for human signal-transduction related diseases and to identify novel therapeutic targets. The Department consists of 14 research groups, maintaining close contacts with other departments of the KU Leuven and with the University Hospital.

The LIPIT laboratory conducts high quality fundamental research, focusing on the role of peroxisomes in complex cellular processes (e.g., lipid metabolism, ROS/RNS metabolism, organelle dynamics and dysfunctions) in mammals, and aims to provide insight into inherited and other human disorders linked to these organelles via cellular studies and mouse models.